pGLO Lab

In this lab we are using the technique called genetic transformation, in order for us to create a bacteria that glows using a gene that codes for green fluorescent protein. Genetic transformation is used, by inserting  a gene of interest from another organism into another, in order to change an organisms genetic makeup. Today, genetic transformation is used in biotechnology in many ways, some uses of this technique are to create perfect fruits and vegetables. Today in our lab, we are using a green flourecent protien that comes from jelly fish in the dark waters of the ocean, and we are going to take this gene and insert it into a plasmid and then insert it into our bacteria. (A plasmid is a circular DNA molecule capable of self replicating itself and also able to carry genes.)