Enzymes and Biofuels

Background info:
 General information about Enzymes: Enzymes help speed up the process of chemical reactions, and are able to work again and again, converting reactants into products, since they do not react with substrat.(Substrat is the reactant in an enzyme-catalyzed reaction.)
Cellulase: Cellulase is the structural polysaccharide found in the cell walls of plants, that is only contained in plants because it's the source of sugars for the plant. Though animals cannot produce cellulose, some animals that eat plants, have a bacteria that produces cellulose. For instance cows produce cellulose because they have a bacteria called Bacteriodes succinogenes, that produces cellulose for them.
Cellulose practical uses: Cellulose today, is used in the bio fuel industry to create ethanol that can help run cars, planes and more. Cellulose is converted into ethanol by extracting the cellulose from a plant's cell wall then converting it into a sugar, and finally the product is changed to ethanol through microbial fermentation.

Purpose: Our purpose for this experiment is to gain more research about the reaction rate in the presence of an enzyme.

Lab instructions:
First we label five curevttes E1-E5, then we put stop solution in all the curvettes.  Because we are adding the stop solution, it will dentaure the enzyme reaction, which will cause p- nitrophenal to turn yellow. Since this solution will turn yellow, it will show how much of a reaction is happening. After this, we will then add substrate to both the enzyme reaction tube and the controlled tube. Then, we will add a buffer to the controlled test tube and then put this solution in the curvette labeled E1. After, we then add some of the enzyme reaction solution to the curvette and then start our timers. After this, we will then add more enzyme reaction to each curvette at set times. On day two of the lab, we used a mushroom as our enzyme instead, by extracting the enzyme in the mushroom with the centrifuge.

Hypothesis and Results: I think the curvettes that have had the longest length in contact with the reaction solution, will be a more darker yellow, while the ones that weren't in contact with the reaction solution, will have a light yellow color. My hypothesis was correct, as we put the solution in the curvette and let it set for a longer time, the more yellow it would turn,which meant more of the product was being produced. Though, we believe our end results were very accurate it, we could have had one mistake, which could have been using dirty curvettes that weren't thoroughly washed from the last period. We also learned that the reaction of the enzymes would soon end because the enzymes would lose recources to make more product, since we saw this in the mushroom lab.